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Figure 1 | BMC Molecular Biology

Figure 1

From: Retroviral vectors for establishing tetracycline-regulated gene expression in an otherwise recalcitrant cell line

Figure 1

Lack of tTA expression prevents β-galactosidase induction in HC11(CMV-tTA/TRE-lacZ) cells. The cells were transiently transfected with the indicated constructs and were cultured either in the presence (A,C,E,G,H,I) or absence (B,D,F,H,J) of 1 μg/ml doxycycline for 48 hours. The CMV-lacZ expression vector was used as a positive control for β-galactosidase expression. The cells were fixed and stained for β-galactosidase expression using X-gal 48 hours after transfection.

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