Figure 4
From: Binding of the baculovirus very late expression factor 1 (VLF-1) to different DNA structures
Effect of magnesium ions on binding of *VLF-1 to three-way junction DNA and cruciform DNA. Reaction mixtures (10 μl) containing 0.004 pmol 32P-labeled oligonucleotide probe and 35 ng of *VLF-1 were incubated for 20 min at 23°C, fixed with 0.5% glutaraldehyde, and analyzed by electrophoresis in a native 6% polyacrylamide gel as described in Materials and Methods. Reactions shown in lanes 1–6 contained three-way junction DNA (substrate IV), those shown in lanes 7–12 contained cruciform DNA (substrate V). No protein was added to the reactions shown in lanes 1 and 7. The final concentrations of MgCl2 (mM) were as indicated.