Table 1 Mobility frequencies of deletions and point mutations in Ll.LtrB ΔDIVb(1–2)
From: Mutations in the Lactococcus lactis Ll.LtrB group II intron that retain mobility in vivo
Deletion or mutation (nucleotides1) | Deletion Size (nucleotides) | Tertiary structure element(s)2 | Mobility Frequency3,4,5 (%) |
|---|---|---|---|
Wild type Ll.LtrB ΔORF 3 | 100 | ||
Domain I : | |||
1. Ia (29–43) | 15 | 1.6 ± 2.1×10-3 | |
2. Ib (46–61) | 16 | α | 0.06 |
3. Ic (65–166) | 102 | ε', θ, β | 3 × 10-3 |
4. Ic1 (70–116) | 47 | ε', θ | <10-3 |
5. Ic2 (122–160) | 39 | β | <10-3 |
6. κ helix (190–204) | 15 | κ | 0.37 |
7. κ helix Id(ii)1 (185–201) | 17 | κ | <10-4 |
8. Helix Id(iii) (205–210,339–346) | 14 | 2 ± 3 × 10-4 | |
9. Id2 (233–255) | 23 | β' | 2.5 ± 2.1 × 10-3 |
10. Id3(i)1 (295–303) | 9 | α' | 2 ± 1.4 × 10-3 |
11. Id4 (316–333) | 18 | 1.5 ± 0.7 × 10-3 | |
12. Ib and Id3(i)1 (46–61, 295–303) | 25 | α, α' | <10-3 |
13. Ic2 and Id2 (122–160, 233–255) | 62 | β, β' | <10-4 |
Domain II: | |||
14. II (395–467) | 73 | θ', η | <10-4 |
15. IIb (432–449) | 18 | 18.26 ± 3.44 | |
16. II(ii)a,b (404–459) | 56 | η | 0.23 ± 0.32 |
Domain III: | |||
17. III (474–523) | 50 | <10-4 | |
18. III(ii),(iii) (486–513) | 28 | <10-3 | |
Domain IV: | |||
19. IVa (538–597) | 60 | 6 ± 4 × 10 -4 | |
20. IVb(1–2) (606–2382) | 158 | 69.7 ± 14.57 | |
Domain V: | |||
21. V (G3A) (2399) | 0.25 ± 0.07 | ||
22. V (G3C) | 4 × 10 -3 | ||
23. V (G3U) | 3 ± 1.4 × 10 -3 | ||
Domain VI: | |||
24. VI (A54 deletion) (2486) | 1 | 3 ± 0.7 × 10 -3 | |
25. VI (A54 G) | 2.02 ± 0.02 | ||
26. VI (A54 C) | 1.12 ± 1.25 | ||
27. VI (A54 U) | 0.67 ± 0.47 |