Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae

BMC Molecular Biology

Table 2 Nonsense mutations in SUP45 lead to decreased ascospore viability

D1628 transformed with plasmids:	Plasmid stability %	Total number of tetrads	Tetrads with indicated number of viable spores				% of His⁺Ura⁺ segregants ^a
			4	3	2	1
pRS316/SUP45	71	49	7	19	22	1	27
pRS316/sup45-101	74	52	6	6	38	2	15
pRS316/sup45-102	71	30	1	1	26	2	5
pRS316/sup45-104	74	48	0	2	42	4	2
pRS316/sup45-105	72	35	0	0	32	3	0
pRS316/sup45-107	69	31	3	5	21	2	14

^a The amount of His⁺Ura⁺ segregants is a characteristic of sup45-n allele presence (His⁺ is the marker for the SUP45 gene disruption and Ura⁺- for the pRS316 plasmid presence). The data correspond to the sum of two experiments but the cell number in nonviable colonies was calculated from only one (Table 3).

ISSN: 1471-2199