Figure 1From: The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cellsMAP kinase activation in WT ES cells. WT ES cells were serum-starved for 36 h and stimulated with 15% FCS or 20 μM LPA for the times indicated. Cell lysates were electrophoresed and immunoblotted with antibodies recognising the phosphorylated forms of ERK1/ERK2, JNK1/JNK2 and p38 (p-ERK1/2, p-JNK1/2 and p-p38). Antibodies against total ERK1/ERK2 and p38 served as loading controls.Back to article page