Table 1 Construct details and shRNA sequences. Vector names and the shRNA sequences they encode. In comments, numbers are coding human PAI-2 mRNA nucleotides (adapted from accession number M18082).
From: Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors
| Â | Hairpin sequence | |||
|---|---|---|---|---|
Name | sense | loop | antisense | Comments |
sh319 | GCGCACACCUGUACAGAUG | CAAGCUUC | CAUCUGUACAGGUGUGCGC | PAI-2 684–702 |
sh321 | GCGCACACCUGUACAGAUGAU | CAAGCUUC | AUCAUCUGUACAGGUGUGCGC | PAI-2 684–704 |
sh323 | GCGCACACCUGUACAGAUGAUGU | CAAGCUUC | ACAUCAUCUGUACAGGUGUGCGC | PAI-2 684–706 |
sh325 | GCGCACACCUGUACAGAUGAUGUAC | CAAGCUUC | GUACAUCAUCUGUACAGGUGUGCGC | PAI-2 684–708 |
sh319scr | GUCAUACCGGCAAGGAUCC | CAAGCUUC | GGAUCCUUGCCGGUAUGAC | scrambled sh319 |
sh325scr | GGCCGGAGAUAAGUUCACUCAACUC | CAAGCUUC | GAGUUGAGUGAACUUAUCUCCGGCC | scrambled sh325 |
sh119 | GAAGACCAGAUGGCCAAGG | CAAGCUUC | CCUUGGCCAUCUGGUCUUC | PAI-2 151–169 |
EGFP | No hairpin, empty vector. | none | ||
U6PT | Human U6 promoter/terminator, no hairpin. | none | ||