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Table 3 Percentage GFP positive cells and mean GFP fluorescence in sh119-transduced IS-1 and IS-1 PAI-2 cells. Transduced cells were assessed for GFP expression by flow cytometry. GFP positive cells were gated equally for each cell type, 5 and 31 days after transduction with the sh119 vector. Mean F is the mean fluorescence of gated GFP positive cells.

From: Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors

 

% GFP positive cells

Mean F of GFP positive cells

 

Day5

Day31

Day5

Day31

IS-1 cells

85

40

146

83

IS-1 PAI-2 cells

82

46

180

96