Figure 4

Silencer activity of RAMP-1 repressor element in transfected HEK293 cells. A RAMP-1 repressor element fragment (-783 to -343) was generated by PCR and ligated directly 3' to the luciferase gene in the RAMP-1 expression plasmid -343 RAMP-1/luciferase. The fragment was oriented in two different positions (5' to 3' and 3' to 5'). Relative luciferase activity was compared between these constructs, pGL3-basic, a plasmid containing 470 and 4.7 kb of RAMP-1 5' flanking region. Three independent sets of transfections were performed each giving similar results