Table 3 Candidate reference genes for normalization of qRT-PCR ranked according to their expression stability (calculated as the average M value after stepwise exclusion of worst scoring genes) by the geNorm VBA applet.
Ranking Order | Gene Name | Average M value |
|---|---|---|
1/2 | GAPDH/YWHAZ | 0.338 |
3 | RPS18 | 0.416 |
4 | RPL4 | 0.469 |
5 | SDHA | 0.517 |
6 | Act-B | 0.563 |
7 | TFRC | 0.604 |
8 | PGK1 | 0.638 |
9 | HPRT1 | 0.671 |
10 | B2M | 0.732 |