Table 2 Sequence information for primers and probes used.
Gene | Oligo | Exon boundary | Sequence – 5' to 3' |
|---|---|---|---|
ACTB | Assay by design (ABI) | ||
AHSP | AHSP Forward | 2 to 3 | CTGAATCAGCAGGTCTTCAATGATC |
AHSP Reverse | GTCCCTGTACTTGGCCAGGA | ||
AHSP Probe | FAM-TCGTCTCTGAAGAAGACATGGTGACTGTGG -TAMRA | ||
B2M | B2M Forward | GAGTATGCCTGCCGTGTG | |
B2M Reverse | AATCCAAATGCGGCATCT | ||
B2M Probe | 2 to 4 | FAM-CCTCCATGATGCTGCTTACATGTCTC-TAMRA | |
HBG2 | HBG2 Forward | CTGCACTGTGACAAGCTGCAT | |
HBG2 Reverse | CGAAATGGATTGCCAAAACG | ||
HBG2 Probe | 2 to 3 | FAM-CCAGCACATTTCCCAGGAGCTTGAAGT-MGB | |
GAPDH | Assay by design (ABI) | ||
HBS1L | HBS1L Forward | AAGGACAAGAATGAGGCAACAGTAT | |
HBS1L Reverse | TTTGGCACAATTTCAGATTCACTT | ||
HBS1L Probe | 4 to 5 | FAM-AGCAAAAGGAAAACCAGTAGATTCCCAGACATC-TAMRA | |
HPRT1 | Assay by design (ABI) | ||
SDHA | Assay by design (ABI) |