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Figure 1 | BMC Molecular Biology

Figure 1

From: Appropriate 'housekeeping' genes for use in expression profiling the effects of environmental estrogens in fish

Figure 1

Expression of the candidate 'housekeeping' genes in fathead minnow liver following exposure to 10 ng EE 2 /L. (A) 18S rRNA, (B) rpl8, (C) ef1a, (D) g6pd, (E) bactin, (F) gapdh, (G) hprt1, and (H) tbp. The results are represented as means ± S.E.M. and expressed as fold-increase in expression from the control which was arbitrarily set as the 1× expression of the gene. Each treatment group consisted of eight male and eight female fish and each fish was analysed in triplicate. Statistically significant differences in gene expression between control and EE2-treated fish for each sex are denoted as follows: * P < 0.05, ** P < 0.01, *** P < 0.001 (Student's t-test).

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