Figure 4

Detection of a 40 nucleotide sequence in the male specific satellite I repeat and a 32 nucleotide sequence in the LPA gene. Padlock probes were hybridized and ligated to pre-treated chromosomes, followed by target primed rolling circle DNA synthesis. (A) Site specific detection of a 40 nucleotide sequence in the male specific satellite I region on the long arm of the Y-chromosome. (B) Site specific detection of a 32 nucleotide sequence of the kringle IV domain in the LPA gene. It seems that individual rolling circle reactions can produce products long enough to be resolved as threads of DNA in the microscope. Scale bar, 100 μm.