Figure 2

Genome-wide microarray analysis of DNA re-replication over time in wild-type cells. The relative amplification of sequences was determined by competitive hybridization of DNA from re-replicating cells against DNA from the same strain prior to re-replication. DNA re-replication profiles of wild-type cells induced for 17 hours, 21 hours, and 25 hours are shown for chromosomes 1 (A), 2 (B), and 3 (C). Centromeres are indicated by yellow squares. No smoothing of data has been applied. Vertical lines indicate the relative level of DNA amplification across the genome. A value of 1.0 is the average amount of re-replicated DNA for the genome. Values greater than 1.0 represent probes which were replicated more than the average amount of re-replication. The results reveal a gradual increase in differences between the most amplified and least amplified regions over time.