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Figure 1 | BMC Molecular Biology

Figure 1

From: Ets-2 and C/EBP-beta are important mediators of ovine trophoblast Kunitz domain protein-1 gene expression in trophoblast

Figure 1

Detection of ovTKDP-1, IFN-τ, Ets-2 and C/EBP-β mRNA in sheep trophectoderm. RT reactions were performed on sheep trophoblast RNA collected from different days of pregnancy. After binding to oligo dT primer, reverse transcription and PCR amplification with primers specific for each message were performed. A) Comparison of ovTKDP-1 and IFN-τ expression during the peri-implantation period of conceptus development. Lanes 1–3: positive control PCR with TKDP-1, IFN-τ and S25 plasmid templates, respectively; lanes 4–9: PCR with days 14, 15, 16, 17, 19 and 25 sheep trophoblast cDNA. B) Ets-2 mRNA expression during the peri-implantation period of conceptus development. Lane 1: DNA marker; lane 2: positive control PCR with Ets-2 expression plasmid; lanes 3–8: PCR with day 14, 15, 16, 17 and 25 cDNA. C) C/EBP-β mRNA expression;. Lane 1: DNA marker; lane 2: positive control PCR with C/EBPβ expression plasmid; lanes 3–8: PCR with day 14, 15, 16, 17 and 25 cDNA.

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