Figure 7
Competition EMSA with recombinant Ets-2 protein and a 32P-labeled ds Ets-2 consensus oligonucleotide. Lane 1: labeled oligonucleotide+Ets-2-GST-fusion protein; lane 2: labeled oligonucleotide+GST protein; lane 3: labeled oligonucleotide+Ets-2-GST-fusion protein+anti-Ets-2 IgG; lane 4: labeled oligonucleotide+Ets-2-GST-fusion protein+unlabeled Ets-2 consensus oligonucleotide (250-fold molar excess); lanes 5–10: labeled oligonucleotide+Ets-2-GST-fusion protein+unlabeled overlapping oligonucleotides from the Tkdp-1 promoter (-150 to +20) at 250-fold molar excess.