Figure 8

Effects of C/EBP-α, -β and -δ on the Tkdp-1 140 bp promoter activity in JEG-3 cells. Luc expression driven by the minimal promoter in cells co-transfected with each of the C/EBP isoforms A) in the absence and presence of over-expressed Ets-2 and B) in the presence of over-expressed Ets-2 and 250 μM 8-BrcAMP. Luc activities are expressed relative to that of the basal activity of the 140 bp promoter and data are shown as mean ± SEM (n = 3). The following values are significantly different at P < 0.05: A) 140 vs. Ets-2, C/EBP-α+Ets-2, C/EB-Pβ+Ets-2, C/EBP-δ+Ets-2 and B) 140 vs. cAMP+Ets-2, cAMP+Ets-2+C/EBP-α, cAMP+Ets-2+C/EBP-β, cAMP+Ets-2+C/EBP-δ; cAMP+Ets-2 vs. cAMP+Ets-2+C/EBP-β, cAMP+Ets-2+C/EBP-δ. cAMP+Ets-2+C/EBP-β is significantly different (P < 0.05) from both cAMP+Ets-2+C/EBP-α and cAMP+ Ets-2+C/EBP-δ.