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Figure 9 | BMC Molecular Biology

Figure 9

From: Ets-2 and C/EBP-beta are important mediators of ovine trophoblast Kunitz domain protein-1 gene expression in trophoblast

Figure 9

Effects of the CCAAT/enhancer element (-117 to -109) mutation on the Tkdp-1 140 bp promoter activity in A) JEG-3 and B) NIH3T3 cells. A) Basal, activated PKA, Ets-2, Ets-2+activated PKA-dependent activation of the wild type and C/EBP-mutated minimal promoter in JEG-3 cells. B) Basal, activated Ras, Ets-2, Ets-2+activated Ras-dependent activation of the wild type and C/EBP-mutated promoter in NIH3T3 cells. Luc activities are expressed relative to that of the basal activity of the 140 bp promoter and data are shown as mean ± SEM (n = 3). Following values are significantly different at P < 0.05: A) 140+PKA vs. C/EBPmut140+PKA; 140+Ets-2 vs. C/EBPmut140+Ets-2; 140+Ets-2+PKA vs. C/EBPmut140+Ets-2+PKA and B) 140+Ets-2 vs. C/EBPmut140+Ets-2 and 140+Ets-2+Ras vs. C/EBPmut140+Ets-2+Ras.

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