Figure 3

Relative abundance of alternatively- and canonically-spliced mfl mRNA subforms. The abundances of the alternatively- and canonically- spliced mfl mRNAs were measured by quantitative real-time RT-PCR in poly(A)+ RNA from adult Drosophila females, manually-dissected ovaries and cultured S2 cells. Three different RNA extractions were examined for each sample, and each reaction was performed in triplicate. Data were normalized to αTub84B expression and are presented relative to the female sample; they represent three independent experiments.