Skip to main content
Figure 4 | BMC Molecular Biology

Figure 4

From: The coding/non-coding overlapping architecture of the gene encoding the Drosophila pseudouridine synthase

Figure 4

Amino acid sequence and expression of the novel MFLα protein subform. (A) Alignment of MFL (top), dyskerin (middle) and MFLα (bottom) amino acid sequences. White letters highlight identical amino acids on a black background, and different but conserved amino acids on a grey background; block letters on a white background indicate different and non-conserved residues. Lines above or below the sequences indicate putative functional domains. NLS: nuclear localisation signal; TruB I, Trub II: regions having homology with members of the bacterial tRNA pseudouridine synthase family; tyr: tyrosine domain containing a putative uracil binding pocket; PUA: a conserved RNA binding domain observed in archaeal, bacterial and eukaryotic RNA modifying proteins. (B) Western blot analysis of extracts from S2 cells. Affinity-purified rabbit polyclonal antibodies recognizing both MFL and MFLα were utilised (see Methods). A major band corresponding to the MFL subform (56 kDa) and a minor band corresponding to the MFLα subform (29 kDa) were detected. Positions of the protein ladder (Precision Plus Prestained Protein Standard Dual Colour; Biorad Laboratories) are shown on the left.

Back to article page