Figure 4From: Leptin induces inflammation-related genes in RINm5F insulinoma cellsSignalling capacity of RINm5F cells expressing LEPRb point mutants. RINm5F cells stably expressing the wild type LEPRb (YYY) or the indicated LEPRb tyrosine/phenylalanine mutants were treated with 100 ng/ml leptin (L) or vehicle (∅) for 15 min before total cell lysates were prepared. Leptin-induced phosphorylation of downstream signalling molecules was assayed by Western blotting and immunodetection with phospho-specific antibodies.Back to article page