Figure 8From: Regulation of poly(ADP-ribose) polymerase-1 (PARP-1) gene expression through the post-translational modification of Sp1: a nuclear target protein of PARP-1rPARP-1 promoter activity in PARP-1+/+ and PARP-1-/- cells. (A) The recombinant plasmids PCR3 and PCR3F2/F3/F4m were transfected into both PARP-1+/+ and PARP-1-/- cells grown with or without the PARP-1 inhibitor PJ34. CAT activities were measured and normalized to the amount of hGH secreted into the culture medium. Values are expressed as ((%CAT activity/100 μg proteins)/ng hGH). Asterisks (*) indicate CAT activities from cells exposed to PJ34 that are statistically different from those measured when cells are transfected with pCR3 in the absence of inhibitor whereas †corresponds to CAT activities in PARP-1-/- cells that are statistically different from those measured in PARP-1+/+ cells (P < 0.005; paired samples, t-test). S.D. is also provided. (B) The plasmids PCR3, -92α5CAT and α6–84 were transfected into both PARP-1+/+ and PARP-1-/- cells and CAT activity (expressed as the ratio of CAT activity from PARP-1-/- cells over that measured in PARP-1+/+ cells (considered as 100%)) measured and normalized as detailed above. Asterisks (*) correspond to CAT activities in PARP-1-/- cells that are statistically different from those measured in PARP-1+/+ cells (P < 0.005; paired samples, t-test).Back to article page