Figure 1
From: Ror2 modulates the canonical Wnt signaling in lung epithelial cells through cooperation with Fzd2
Effects of Wnt3aCM and Wnt5aCM on Canonical Wnt signaling in L, A549, and H441 cells. Cultured L, A549, or H441 cells were transfected with pSV-β-gal (Promega, WI) and STF plus Ror2WT (+Ror2) or the control vector (-Ror2). Twenty four hours after transfection, cells were treated with L-CM, Wnt3aCM or Wnt5aCM. Wnt3aCM and Wnt5aCM were used at 1/8 and 1/4 dilutions, respectively. Equal amount of conditioned media was used in each assay. Luciferase values representing the STF activities were normalized to beta-galactosidase for transfection efficiency. Values of the assays treated with L-CM alone (#1 and #5) were adjusted to unity to normalize all other experimental values in the same group. CM: conditioned medium. * indicates p < 0.05.