Figure 7From: Transcriptional regulation of BRD7 expression by Sp1 and c-MycKnockdown of endogenous c-Myc increases the promoter activity and mRNA transcription of BRD7 gene. (A) Detecting of c-Myc expression level in 5-8F/Si-c-Myc and 5-8F/Si-control cells by immunoblot analysis. The anti-c-Myc was used as primary antibody. The expression of α-tubulin was used as an internal control. (B) Luciferase assay of pGL3/-266,-212 promoter activity in 5-8F/Si-c-Myc and 5-8F/Si-control cells. Luciferase activity was measured in cell extracts 38 h after transfection. The SV40 β-galactosidase vector was used for normalizing transfection efficiency. Data are the means ± S.D. of three independent experiments. (C) Detection of BRD7 expression in 5-8F/Si-c-Myc and 5-8F/Si-control cells by RT-PCR. The PCR products were analyzed by electrophoresis on a 2%Back to article page