Figure 3
![Figure 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1471-2199-9-12/MediaObjects/12867_2007_Article_269_Fig3_HTML.jpg)
Identification of SUMO1 conjugation site in CoCoA. (A) COS-7 cells were co-transfected with plasmids expressing EGFP-SUMO1 (200 ng), PIAS1 (100 ng), and full-length HA-CoCoA wild type or mutants (800 ng) as indicated. (B) 293T cells were transfected with plasmids expressing EGFP-SUMO1 (200 ng), PIAS1 (100 ng), and HA-CoCoA(1–190) wild type or K29R mutant (800 ng) as indicated. Cell extracts were collected 48 h after transfection and analyzed by immunoblot with anti-HA antibody. (C) COS-7 cells were co-transfected with plasmids expressing Myc-SUMO2 (200 ng), PIAS1 (100 ng), and full-length HA-CoCoA wild type or mutants (800 ng) as indicated. Cell extracts were collected 48 h after transfection and analyzed by immunoblot with anti-HA antibody.