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Table 1 PCR primers for the amplification of reference gene candidate genes, designed with the software Primer Express 2.0 (Applied Biosystems).

From: Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus

Reference gene

 

Orientation

5'- 3' Sequence

Bp

Tm (°C)

Beta-Actin

actb

For

GCGTGGCTACTCCTTCACC

19

57

  

Rev

AGGACTTCATACCGAGGAAGG

21

56

Eucaryotic elongation Factor 1 alpha

eef1a

For

CCACCGTTGCCTTTGTCC

18

58

  

Rev

TGGGACTGTTCCAATACCTCC

21

57

Hypoxanthine phosphoribosyltransferase 1

hprt1

For

GACGCAGATATGGTTCAGATCTC

23

57

  

Rev

GTCTTGATTGTGGAGGATATTATCG

25

57

L13A ribosomal binding protein

rpl13a

For

CACCTTGGTCAACTTGAACAGTG

23

58

  

Rev

TCCCTCCGCCCTACGAC

17

58

RNA-Polymerase II

rpo2

For

TTAACAGGTGGGGGGTGC

18

58

  

Rev

AGCTCAAGAGCAGAAAGATCCC

22

58

TATA-Box binding protein

taf2

For

GGAGTTCATGTTGCAGGTTTTC

22

57

  

Rev

CGTTCCTCTTCCTATTGAAGGC

22

58

Ubiquitin

ubc

For

AGACGGGCATAGCACTTGC

19

58

  

Rev

CAGGACAAGGAAGGCATCC

19

57

Glyceraldehyd-3-phosphate dehydrogenase

gapd

For

TGGTCAGCTTACCGTTGAGC

20

58

  

Rev

CCCCCTGGCTAAAGTCATCC

20

59

Beta-2-microglobulin

b2m

For

AGACTATGCCTGGGAATCAAAC

22

56

  

Rev

GAAGATGTGTTGAATAGAAGCTGG

24

56

18S rRNA

18S rRNA

For

GACTCCGGTCCTATTTTGTGG

21

57

  

Rev

GCTAGTTGGCATCGTTTATGG

21

56

Glucose-6-phosphate dehydrogenase

g6pd

For

GGACGGTGTTCCTTTCATCC

20

58

  

Rev

TAGGTGAGGTCCAGCTCGG

20

57

Peptidylprolyl isomerase A

ppia

For

CCCCTGGCTGGACGG

15

58

  

Rev

TAAAAATGACGGGAGGGGG

19

58