Figure 4
From: HMG-box domain stimulation of RAG1/2 cleavage activity is metal ion dependent
Tailless forms of HMGB1 stimulate RAG-mediated 23-RSS cleavage at lower concentrations than full-length HMGB1, and BA Tailless exhibits a selective impairment in facilitating site-specific nicking by the RAG complex. (A-B) In vitro cleavage reactions were performed as in Fig. 2, except that BA Tailless and AB Tailless replaced HMG-box A and HMG-box B in the cleavage reactions.