Figure 3
From: Validation of internal control for gene expression study in soybean by quantitative real-time PCR
Determination of the optimal number of HKG for normalization by pairwise variation using geNorm. Pairwise variation (V) to determine the optimal number of HKG(s) for accurate normalization in all 21 sample pools (A), different tissues at the same developmental stage (B), developmental series (C), photoperiod treatment (D), different cultivars (E), different time of the day series (F), leaves on different nodes on the main stem (G).