Figure 8
![Figure 8](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1471-2199-9-86/MediaObjects/12867_2008_Article_343_Fig8_HTML.jpg)
K m determination for etheno-NAD+ of chicken ART4. Recombinant chART4 (7 μg/ml) was incubated at room temperature for 0.5, 1.5, 2.5, 3.5, 4.5, 5.5, 6.5, 8, 10, 12, 15, 20, and 30 min in the presence of poly-L-arginine (1 mg/ml) and 0.5, 1, 2, and 5 μM etheno-NAD+ in the presence (open circles) and absence (filled circles) of PNGase F. The ADP-ribosyltransferase activity was determined by measuring the increase in fluorescence intensity. Shown is a double reciprocal plot of the data of the dose response. Data show one representative experiment out of three (Km "untreated" vs. Km "treated" chART4 p = 0.062, Student's t-test).