Figure 4
![Figure 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2F1471-2199-9-92/MediaObjects/12867_2008_Article_349_Fig4_HTML.jpg)
Polypyrimidine tracts and polypyrimidine tract binding protein (PTB) regulate the expression of p11-CT. A) Western blot of wild type Cx55.5-EGFP (WT) and PPT1 and PPT2 deleted (WT-PPTDel3) fusion constructs transiently transfected in N2A cells in the presence of either endogenous PTB (-) or overexpressed PTB (+). Immunodetection was performed by using anti-GFP antibody as described above. B) Western blot detection of endogenous and huPTB using anti-PTB antibody as described above and with β-actin as loading control.