Figure 2

H ₂ O ₂ induced apoptosis and upregulated miR-100 in RGC-5 cells. RGC-5 cells were treated with various concentrations of H₂O₂ for 24 hours to induce apoptosis. (A) Representative fluorescent images were shown for the untreated RGC-5 cells (control), and RGC-5 cells treated with 400 μM or 1000 μM H₂O₂. An antibody against Brn3a (blue) was used to identify RGC-5 cells, and TUNEL staining was applied to identify apoptotic cells among them. (B) Quantification of the percentage of apoptotic RGC-5 cells (*: P <0.05, as compared to control). (C) The expression levels of miR-100, corresponding to the application of various concentrations H₂O₂, were measured by qRT-PCR (*: P <0.05, as compared to control).