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Figure 2 | BMC Molecular Biology

Figure 2

From: Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins

Figure 2

Expression and purification of r3xFNLDD-D. (A) Coomassie Brilliant Blue (CBB) staining of the recombinant protein. The purified proteins were subjected to SDS-PAGE and CBB staining. (B) Immunoblot analysis (IB) of r3xFNLDD-D. The purified proteins were subjected to SDS-PAGE and IB with anti-Dock Ab. Supernatant: the supernatant prepared from the silkworm pupal homogenates. Precipitant: the insoluble precipitate prepared from the silkworm pupal homogenates. Elution: the eluate after affinity purification with Dock Catch Resin.

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