Figure 6

Myh foci induced by oxidative stress and Sirt6 depletion are frequently localized on telomeres. a, b Normal (WT) MEF cells; c, d sirt6 ^−/− (knockout, KO) MEF cells. b, d Cells were treated with 0.3 mM H₂O₂ for 1 h and recovered for 4 h. Telomere FISH (T-FISH) in red, immunostained mMyh in green, DAPI-stained DNA in blue. Arrows indicate yellow mMyh foci localized on telomeres. e Quantitation of the number of mMyh foci per nucleus. f Quantitation of percentage of mMyh foci on telomeres [yellow/(green + yellow) in 3rd columns of (b–d)]. Experiments were repeated twice and more than 6 images were analyzed. Error bars indicate SD; n ≥ 6. The data of wild-type cell without oxidative stress is not included because the number of mMyh foci is very few [(a, e)]. Two stars indicate that p values are smaller than 0.05.