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Fig. 1 | BMC Molecular Biology

Fig. 1

From: Analysis artefacts of the INS-IGF2 fusion transcript

Fig. 1

RNA sequencing read depth in the INS/IGF2 genomic region. Plot of the genomic region of chromosome 11 around the INS and IGF2 loci (2150,000–2190,000; genomic assembly: GRCh37/hg19) with probesets from the Affymetix HG-U133A array and the main transcript variants of INS, INS-IGF2 and IGF2 indicated below the plot. All shown transcripts are encoded on the antisense strand (as indicated by the direction of the arrows in the introns)—for an overview of all known transcript variants, please see Additional file 1: Figure S1 (panel A). The y-axis shows the read depth of the RNA sequencing study from Nica et al. [1]—notice that read depth was capped at 8,000 (data shown for beta cell dataset #6—the same pattern was observed for all other data sets). From the plot it is clearly seen that few reads maps to the INS-IGF2 exons in the 2170000 area (middle of the plot) and almost all signal assigned to INS-IGF2 by Nica et al. [1] originates from the first coding exon which is shared with INS (right hand side of the plot). All positional information on this plot is from ENSEMBL v.75.

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