Fig. 4
From: Deciphering targeting rules of splicing modulator compounds: case of TG003
Difference in response to TG003 is derived from difference in sequence and not trans-environment. Left RT-PCR of endogenous mouse Dennd4c exons 28–31 and transfected human DENND4C exons 26–29 in pEGFPc1-human-DENND4c-e29–31 transfected C2C12 cells. Right RT-PCR of endogenous human DENND4C exons 25–29 and transfected mouse Dennd4c exons 29–31 in pEGFPc1-human-DENND4c-e29–31 HEK293 cells. After transfection both experiments were treated with 50 µM of TG003 for 4 h before harvest