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Fig. 1 | BMC Molecular Biology

Fig. 1

From: Transfection of Sertoli cells with androgen receptor alters gene expression without androgen stimulation

Fig. 1

Expression of androgen receptor (Ar) mRNA in Sertoli cell cultures. To find an appropriate cell culture system for our planned transfection studies, RT-PCR with specific primers for mouse and rat Ar was performed. Testis homogenate from rat and mouse served as positive control, whereas water was used as no template control (NTC) samples. We tested two mouse (WL3 and SK-11) as well as two rat Sertoli cell lines (SCIT-C8 and 93RS2). The latter revealed no expression of intrinsic Ar and were therefore used for further experiments

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BMC Molecular Biology

ISSN: 1471-2199

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