Fig. 3
a Distribution of Rad9 in the chromatin-bound fraction in Wt and Mut TLK1B expressing cells in response to treatment and recovery from HU. Values are mean ± standard error for n = 3. b Distribution of Rad9 in the cytoplasmic fraction in Wt and Mut TLK1B expressing cells. Note that the Rad9 in the cytoplasm appears as a single band, but in the chromatin fraction appears as multiple bands. Rad9 gets multiply phosphorylated only once it gets loaded onto the chromatin. Values are mean ± SE for n = 3. c Distribution of p-Rad9 (S328) in the cytoplasmic fraction in Wt vs Mut TLK1B expressing cells. Note that p-Rad9 (S328) is predominantly cytoplasmic and is elevated in Mut TLK1B expressing cells during replication stress. Values are mean ± standard error for n = 3. d Distribution of Rad9 in the chromatin-bound fraction in HEK293 cells in response to treatment and recovery from HU in the presence or absence of TLK inhibitor (THD)