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Fig. 4 | BMC Molecular Biology

Fig. 4

From: TLK1B mediated phosphorylation of Rad9 regulates its nuclear/cytoplasmic localization and cell cycle checkpoint

Fig. 4

a Verification of fractionation procedure by probing for ORC2 which is chromatin-bound and tubulin which is cytoplasmic. b α-Flag Ab detects only the overexpressed flag tagged Wt and Rad9 (S328D) mutant. c α-Rad9 Ab detects both the endogenous and the overexpressed Wt and Rad9 (S328D) mutant. d Immunolocalization shows that the Rad9 (S328D) mutant localizes primarily in the cytoplasm in contrast to the WT protein which is mostly nuclear

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