Fig. 1From: Immunoblot screening of CRISPR/Cas9-mediated gene knockouts without selectionsgRNA/Cas9-mediated gene knockouts. sgRNA/Cas9 targeting to a gene exon induces a double-stranded DNA break that is repaired by non-homologous end joining, often introducing frameshift mutations (depicted in red) that lead to lack of functional protein production and likely cause nonsense-mediated decay of the mRNABack to article page