Fig. 3
From: MicroRNA 433 regulates nonsense-mediated mRNA decay by targeting SMG5 mRNA
MiR-433 reduced SMG5 protein and mRNA level. a Western Blot analysis of endogenous SMG5 protein levels in BHK cells 48 h after transfection with mature miR-433 mimic or a random negative-control miRNA mimic (in C2C12 cells 48 h after transfection with miR-433 inhibitor or a random negative-control). β-actin was used as the internal control. b Quantitative polymerase chain reaction (qPCR) analysis of BHK cells transfected for 48 h with the miR-433 mimic or inhibitors (in C2C12 cells). Shown are the results from three independent experiments, normalized to β-actin mRNA levels. **P < 0.01