Fig. 5

The N-terminal fragments of p400 interfere with the protein–protein interaction between ATM and p400. a HEK293T cells virally transduced by either control or F1 fragment expressing lentivirus were selected against puromycin and then used for the transient expression of HA-p400 in duplicate. The cell lysates were pre-cleared by protein A/G beads for 3 h and subjected to immunoprecipitation by anti-HA antibody-conjugated beads. Two percent of prepared cell lysates was used as input to ensure comparable expression levels of ATM, TRRAP and p400 in control (C) and Flag-F1 fragment (F1) cell lines. The immunoblot signals from co-immunoprecipitated ATM and TRRAP were quantified using Image J software and the ratios of ATM to TRRAP were calculated (ATM/TRRAP). b The same experiments were conducted except the transient expression of F2 and F3 fragments in HEK293T cells. The data represents one of two independent experiments with similar results