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Fig. 2 | BMC Molecular Biology

Fig. 2

From: 3C-digital PCR for quantification of chromatin interactions

Fig. 2

Representative duplex 3C-dPCR plots in cell line DU-145. a EcoRI digestion efficiency test, 197 copies of undigested EcoRI fragment per 1000 genome using a primer pair across the EcoRI site 2L at 2q11.2 loci. b Strong interaction signal for self-ligation fragment by the primer designed on the same fragment with anchor primer and paired with the anchor primer at 2q11.2, 44 copies per 1000 genome. c Moderate interaction signals for nearby ligation by the adjacent primer designed on the fragment next to the anchor primer at 2q11.2, 3.6 copies per 1000 genome. d Relative weak interaction signal for long-distance interaction between 2L with EcoRI fragment T7 covering the promoter of gene CAPG, 1.7 copies per 1000 genome. The x-axis displays the amplitude of genome copy number control (labeled by HEX, red) and the y- axis is signal strength of target ligation products (labeled by FAM, blue). The signals in the lower left quadrant are negative for both targets (yellow). The signals in the upper right quadrant are positive for both targets (green)

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