Fig. 2
From: Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5
Exogenous S-SOX5 up-regulates SPAG16L mRNA expression in BEAS-2B cells. a Western blot analysis of S-SOX5 protein levels in BESA-2B cells stably expressing S-SOX5 (left) or infected with AdS-SOX5 (right) detected with the Pico system. b Analysis of SPAG16L mRNA expression by real-time PCR. GAPDH was also amplified simultaneously to normalize the results. Values indicate mean ± S.E. (error bars) of the relative mRNA level (n = 3). *p < 0.05 (Student’s t test) compared to the controls