Fig. 4
From: Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5
S-SOX5 associates with the human SPAG16L promoter as revealed by ChIP assay. A Schematic representation of the human SPAG16L proximal promoter and the regions (a–c) amplified by ChIP primers used in this study. Arrows show the location of the primers. B Representative ChIP assay results with BEAS-2B cells infected by AdS-SOX5 using a control rabbit IgG or an antibody specifically against SOX5. a with a primer set not flanking any potential SOX5 binding sites; b and c, with primer sets flanking potential SOX5 binding sites. C qPCR quantification of ChIP products. DNA recovered from ChIP was used as a template for real-time PCR analysis. Data shown are mean ± S.E. (error bars) of three independent replicates. *p < 0.05 (Student’s t test) compared to the normal rabbit IgG pulldown group