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Fig. 6 | BMC Molecular Biology

Fig. 6

From: Methylated site display (MSD)-AFLP, a sensitive and affordable method for analysis of CpG methylation profiles

Fig. 6

Sensitivity of MSD-AFLP analysis. ac Scatter plots of CpGs where MSD-AFLP analysis showed statistically significant differences between two tissues as determined by one-way ANOVA using Benjamini–Hochberg corrections followed by the post hoc Tukey test. Tissue data is derived from the average of three mouse specimens. Significant differences in methylation level were found in the 592 CpGs between the hippocampus and the liver (a), 641 CpGs between the hippocampus and the kidney (b), and 457 CpGs between the kidney and the liver (c). X-axis, absolute values of the difference in percent methylation level; y-axis, absolute values of the ratio of percent methylation level. H hippocampus; L liver; K kidney. Note that a, 9 CpGs; b, 8 CpGs; and c, 7 CpGs were found to be statistically significant in both differences in percent methylation levels of less than 5% and ratios of percent methylation level of less than 1.1-fold. d Example of slight but statistically significant difference in percent methylation level (Chr.17 35946553 and Chr.10 83903974) between two tissues derived from MSD-AFLP data. Student’s t test. *p < 0.05. e Confirmation of statistically significant difference in methylation level d by analyzing the same CpG site using MSRE-PCR with locus-specific primers. Student’s t-test. *p < 0.05

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