Table 1 List of primers used for bisulfite PCR and qRT-PCR
From: RUNX1 induces DNA replication independent active DNA demethylation at SPI1 regulatory regions
Bisulfite primers | |
SPI1 proximal promoter (Fwd) | GAGATTTTTTGTATGTAGTGTAAGA |
SPI1 proximal promoter (Rv) | TAACTTCCCACTAATAACAAACCA |
SPI1 −17-kb URE (Fwd) | GTTGGATATTTTTTTGAGGTTTGG |
SPI1 −17-kb URE (Rv) | TAAAACCTAAAACTACTAAACCCA |
Real-time primers | |
GAPDH (Fwd) | GAAATCCCATCACCATCTTCCAGG |
GAPDH (Rv) | GAGCCCCAGCCTTCTCCATG |
SPI1 (Fwd) | TCCTGAGGGGCTCTGCATT |
SPI1 (Rv) | TGTCATAGGGCACCAGGTCTT |