Fig. 7
Representative plots of real-time PCRs using literary primer pairs specific for mouse α-SMA. Real-time PCRs using mα-SMAL1, mα-SMAL2 or mα-SMAL3 primer pairs and mα-SMAT DNA templates resulted in products (CtL1 = 28.09, CtL2 = 17.19, CtL3 = 16.79) with single melting peaks at 82, 81.9, or 83.3 °C and in discrete bands in the agarose gel at 60, 101 or 154 bp, respectively (a–c). Real-time PCRs using mα-SMAL1, mα-SMAL2 or mα-SMAL3 literary primer pairs amplified the non-specific mß-actinT DNA templates also (CtL1 = 31.09, CtL2 = 34.62, CtL3 = 32.96), resulted in melting peaks at 81, 81.9, or 83.3 °C and in discrete bands at 60, 101 or 154 bp, respectively (a–c)