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Table 2 Statistical analysis of mRNA expression of α-SMA and ß-actin in mice kidneys underwent UUO

From: Selective measurement of α smooth muscle actin: why β-actin can not be used as a housekeeping gene when tissue fibrosis occurs

Applied primer

Normality test

Comparison test (control vs. UUO)

Test

P value

Test

P value

Control

UUO

mα-SMASD

Kolmogorov–Smirnov

0.2

0.2

Unpaired t test

<0.0001

mα-SMAL1

Kolmogorov–Smirnov

0.0115

0.2

Mann–whitney U test

0.0023

mα-SMAL2

Kolmogorov–Smirnov

0.2

0.2

Unpaired t test

<0.0001

mα-SMAL3

Kolmogorov–Smirnov

0.2

0.2

Unpaired t test

<0.0001

mß-actinSD

Kolmogorov–Smirnov

0.1283

0.2

Unpaired t test

0.4187

mß-actinL1

Kolmogorov–Smirnov

0.2

0.2

Unpaired t test

0.0022

mß-actinL2

Kolmogorov–Smirnov

0.2

0.2

Unpaired t test

0.0047

mß-actinL3

Kolmogorov–Smirnov

0.2

0.2

Unpaired t test

0.0021

  1. The mRNA expression of α-SMA and ß-actin was determined by comparison with the expression of 18S ribosomal RNA (RN18S) as internal control from the same samples using the ∆∆Ct method