Fig. 2From: An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencingComparison of workflow of the ‘old’(classical) and ‘new’ protocol for RNA isolation from deparaffinised, stained and laser-capture microdissected FFPE tissue. a Workflow of the ‘old’ RNA isolation protocol, starting with proteinase K digestion of the sample. b Workflow of the ‘new’ isolation protocol, starting with a focused ultrasonication step to disrupt the tissue prior to proteinase K digestion. Values in brackets indicate the approximate timing for each stepBack to article page