Cresyl violet staining procedure for FFPE tissue sections | |
---|---|
100% Xylene, bath 1 | 5 min |
100% Xylene, bath 2 | 5 min |
100% Ethanol | 30 s |
95% Ethanol | 30 s |
70% Ethanol | 30 s |
dH2O | 10 s |
Cresyl violet (75% Ethanol with DEPC treated dH2O, pH 8.0) | 15 s |
dH2O | 10 s |
70% Ethanol | 10 s |
95% Ethanol, bath 1 | 10 s |
95% Ethanol, bath 2 | 10 s |
100% Ethanol, bath 1 | 30–60 s |
100% Ethanol, bath 2 | 30–60 s |