Fig. 1From: An optimized rapid bisulfite conversion method with high recovery of cell-free DNABinding sites of primers and probe used are underlined [23]. The top strand is the undeaminated DNA sequence. Bottom strand is the deaminated sequence. A solid line between the two strands illustrates no difference in sequence, a dotted line marks the positions of cytosine converted to uracil during deamination, and “+” marks the positions of CpG dinucleotides. Primer sequences can be found in Table 1. The universal primers, MLH1 UF and MLH1 R, contain Inosine at one position eachBack to article page